Journal:

Article Title: TLR2 Expression Is Increased in Rosacea and Stimulates Enhanced Serine Protease Production by Keratinocytes

doi: 10.1038/jid.2010.351

Figure Lengend Snippet: (a, b) P. acnes (1 × 106 CFU per ml) were added to normal human epidermal keratinocytes (NHEKs) cultured in low or high calcium media for 24 hours, and then (a) KLK5 mRNA and (b) KLK5 protein in the culture media were measured. (c–e) Skin explants from Tlr2−/− (gray) and Wt (black) mice were treated with P. acnes 6919 ex vivo. At 24 hours after incubation, (c) KLK5 mRNA, (d) protease activity in the culture media, and (e) Camp mRNA were measured. *P<0.05, **P<0.01, ***P<0.001. (f) Skin samples from Wt and Tlr2−/− mice were biopsied before and 2 hours after chemical irritation. In situ zymography visualized protease activity. Scale bar = 100 μm.

Article Snippet: Propionibacterium are the most abundant microbial inhabitants of facial skin ( Grice et al ., 2009 ), and we selected two clinical isolates of Propionibacterium acnes (ATCC no. 11827 and 6919) to explore their influence on the expression of KLK5.

Techniques: Cell Culture, Ex Vivo, Incubation, Activity Assay, In Situ, Zymography